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HaCaT cells (5,000–10,000/well) were seeded in 96-well plates and incubated for 24 h. Sunitinib or SU12662 (N-Desethyl Sunitinib) dissolved in dimethyl sulfoxide (DMSO), with concentrations ranging from 0.5 to 100 μM (6-wells per concentration) were then added and allowed to incubate for another 24 h. After incubation, media was aspirated and replaced with 50 μL of 2 mg/mL of methylthiazolyldiphenyl-tetrazolium bromide (MTT) dye (Duchefa, Haarlem, the Netherlands) in phosphate-buffered saline (PBS) (Bio-Rad, Hercules, USA) and 200 μL of serum-free HEPES-buffered DMEM media. Plates were then incubated in the dark for 2–3 h at 37 °C. Subsequently, medium was then aspirated and the residual dye was re-dissolved in 25 μL of Sorensen’s buffer (0.1 M glycine and 0.1 M NaCl equilibrated to pH 10.5 with 0.1 M NaOH) and 200 μL of DMSO. Plates were read at 570 nm using the Infinite200 (Tecan, Männedorf, Switzerland). Experiment was performed in triplicate for each compound. Cell viability was expressed as a percentage normalised to the vehicle-treated control.