Ro 32-0432 hydrochloride is an orally available cell-permeable selective inhibitor of PKC enzymes. Moreover, Ro 32-0432 inhibits G protein-coupled receptor kinase 5 activity. Ro 32-0432 prevents T cell-driven chronic inflammatory responses in several rat models. Ro 32-0432 inhibits an antigen-driven T-cell-mediated arthritis in vivo. Treatment of rats with Ro 32-0432 in ischemic stroke decreases the ischemic infarction area, neurological symptoms and associated endothelin B receptor upregulation. Ro 32-0432 attenuates the propagation of nicotine dependence and reduce withdrawal signs possibly by G protein-coupled receptor kinase 5 activation-linked mechanisms.